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By Rodolfo Paoletti, Dr. David Kritchevsky

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Figure 2 illustrates the molar percentages of each of these six glycerides, with varying proportions of S and U. It may be noted here that the unsymmetrical mixed glycerides occur in twice the proportion of their corresponding symmetrical isomers. , SSU and USS (which are chemically indistinguishable), but only one of forming SUS. If the S and U are present in equal proportions we can represent this as shown in Scheme I. ) Structural Investigation of Natural Fats 37 Clearly, if the random theory were true, the calculation of the glyceride composition of any natural fat from its fatty acid composition would be a comparatively simple matter.

1962) have demonstrated with the use of synthetic glycerides. In the case of milk fat, Gander et al. (1961) have 28 COLEMAN adduced evidence to show that the native lipase of milk may be more suitable for hydrolysis studies. F. COMBINATION METHODS 1. Oxidation and Hydrolysis A useful combination of the techniques of oxidation and pancreatic lipolysis has recently been described by Youngs ( 1961 ) as a means for the determination of glyceride composition of mixtures. The method consists of subjecting the fat to an oxidation procedure with periodate and permanganate, similar to that described by Von Rudloff (1956).

There are several difficulties associated with Gunstone's theory. First, the monoglycerides produced from most vegetable fats by pancreatic hydrolysis do not contain exclusively Ci 8 unsaturated acids. Second, the discrepancies between the predicted proportions of various mixed glycerides and those found experimentally are often quite large and, perhaps more significant, are all in the same direction. Thus, of the twenty-three fats containing between 20 and 45% of saturated acids, quoted by Gunstone, the mean deviation of the predicted SU2 content from that observed experimentally is —12%.

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